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dna ladder takara 3422a fixation permeabilization buffer r d systems fc007 glucosamine medchemexpress hy b1125 6 diazo 5 oxygen l noleucine don  (TaKaRa)


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    TaKaRa dna ladder takara 3422a fixation permeabilization buffer r d systems fc007 glucosamine medchemexpress hy b1125 6 diazo 5 oxygen l noleucine don
    Dna Ladder Takara 3422a Fixation Permeabilization Buffer R D Systems Fc007 Glucosamine Medchemexpress Hy B1125 6 Diazo 5 Oxygen L Noleucine Don, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+buffer+d/100+bp+DNA+Ladder/pm40938752-290-85-87
    Average 96 stars, based on 576 article reviews
    dna ladder takara 3422a fixation permeabilization buffer r d systems fc007 glucosamine medchemexpress hy b1125 6 diazo 5 oxygen l noleucine don - by Bioz Stars, 2026-10
    96/100 stars

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    Polymerase Chain Reaction:

    Article Title: Enzyme-assisted high throughput sequencing of an expanded genetic alphabet at single base resolution
    Article Snippet: .. A reaction mixture (total volume 20 μl) was prepared in a 100 μl PCR tube containing unlabeled reverse primer (50 nM), 32 P γ-ATP labeled reverse primer (10 nM), template DNA (300 nM), and PCR master mix (containing TaKaRa Taq polymerase, reaction buffer pH 8.9, and 0.2 mM d(A, T, C, G)TP). ..

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Article Title: Enzyme-assisted high throughput sequencing of an expanded genetic alphabet at single base resolution.
    Article Snippet: .. A reaction mixture (total volume 20 μl) was prepared in a 100 μl PCR tube containing unlabeled reverse primer (50nM), 32P γ-ATP labeled reverse primer (10 nM), template DNA (300nM), and PCR master mix (containing TaKaRa Taq polymerase, reaction buffer pH 8.9, and 0.2mM d(A, T, C, G)TP). ..

    Labeling:

    Article Title: Enzyme-assisted high throughput sequencing of an expanded genetic alphabet at single base resolution
    Article Snippet: .. A reaction mixture (total volume 20 μl) was prepared in a 100 μl PCR tube containing unlabeled reverse primer (50 nM), 32 P γ-ATP labeled reverse primer (10 nM), template DNA (300 nM), and PCR master mix (containing TaKaRa Taq polymerase, reaction buffer pH 8.9, and 0.2 mM d(A, T, C, G)TP). ..

    Article Title: Enzyme-assisted high throughput sequencing of an expanded genetic alphabet at single base resolution.
    Article Snippet: .. A reaction mixture (total volume 20 μl) was prepared in a 100 μl PCR tube containing unlabeled reverse primer (50nM), 32P γ-ATP labeled reverse primer (10 nM), template DNA (300nM), and PCR master mix (containing TaKaRa Taq polymerase, reaction buffer pH 8.9, and 0.2mM d(A, T, C, G)TP). ..

    Article Title: Müller cells under hydrostatic pressure modulate retinal cell survival via TRPV1/PLCγ1 complex-mediated calcium influx in experimental glaucoma.
    Article Snippet: Correspondence X. Liu, Department of Cataract, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: xhualiu@sohu.com and G. Zhang, Department of Fundus Disease, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: zhang-guoming@163.com

    Recombinant:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Purification:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Magnetic Cell Separation:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Blocking Assay:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Peptide Fractionation:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Immunohistochemistry:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Reporter Assay:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Membrane:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Protein Extraction:

    Article Title: The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFPT1 Abcam ab125069; RRID: AB_10975709 PD-L1 Abcam ab205921; RRID: AB_2687878 PD-L1 Abcam ab228415; RRID: AB_2884993 PD-L1 Proteintech 66248-1-lg; RRID: AB_2756526 PD-L2 Proteintech 14922-1-AP; RRID: AB_2122732 CTLA-4 R&D Systems MAB434; RRID: AB_2087648 ICOSLG Proteintech 18251-1-AP; RRID: AB_2878523 VTCN1 Proteintech 12080-1-AP; RRID: AB_2877822 SRP54 Abcam ab154796; RRID: AB_3076262 Integrin α2 Proteintech 24552-1-AP; RRID: AB_2879604 Integrin α3 Proteintech 66070-1-AP; RRID: AB_11042765 Integrin β1 Proteintech 12594-1-AP; RRID: AB_2130085 eIF2α Cell Signaling Technology 5324; RRID: AB_10692650 Phospho- eIF2α Cell Signaling Technology 3398; RRID: AB_2096481 eIF4E Cell Signaling Technology 2067; RRID: AB_2097675 Phospho- eIF4E Cell Signaling Technology 9741; RRID: AB_331677 eIF4G Cell Signaling Technology 2617; RRID: AB_2261977 Phospho- eIF4G Cell Signaling Technology 2441; RRID: AB_2277632 eEF1A2 Abcam AB_3073770 CD45 Cell Signaling Technology 41104; RRID: AB_2799194 CD3 Cell Signaling Technology 17857; RRID: AB_2798790 CD8a Cell Signaling Technology 35467; RRID: AB_2799078 Granzyme B Biolegend 515408; RRID: AB_2562196 Biotin Cell Signaling Technology 7075; RRID: AB_10696897 MYC-tag Proteintech 16286-1-AP; RRID: AB_11182162 FLAG tag Merck F1804; RRID: AB_262044 Alexa Fluor 488 Jackson 711-545-152; RRID: AB_2313584 Alexa Fluor 594 Jackson 711-585-152; RRID: AB_2340621 Anti-rabbit IgG HRP linked antibody Cell Signaling Technology 7074; RRID: AB_2099233 Anti-mouse IgG HRP linked antibody Cell Signaling Technology 7076; RRID: AB_330924 FAK Cell Signaling Technology 3285; RRID: AB_2269034 Phospho-FAK (Tyr397) Absin abs131023; RRID: AB_3713625 Phospho-FAK (Y397) Cell Signaling Technology 8556; RRID: AB_10891442 HRP-Conjugated β-actin Monoclonal Antibody Proteintech HRP-60008; RRID: AB_2819183 4E-BP1 antibody Cell Signaling Technology 9452; RRID: AB_10891442 Phospho-4E-BP1 antibody Cell Signaling Technology 2855; RRID: AB_560835 Matriptase Antibody Cell Signaling Technology 19271; RRID: AB_2798814 Phosphotyrosine Antibody Abcam ab179530; RRID: AB_2909401 Sambucus Nigra Lectin, Biotinylated Vector Laboratories B-1305-2; RRID: AB_3717626 Phaseolus Vulgaris Leucoagglutinin, Biotinylated Vector Laboratories B-1115-2; RRID: AB_3713627 Concanavalin A Polyclonal Antibody, HRP ThermoFisher GA-CONA-PO; RRID: AB_3713628 MZF1 Santa Cruz sc-293218; RRID: AB_3713629 Na,K-ATPase Antibody Cell Signaling Technology 3010; RRID: AB_2060983 (Continued on next page) Cell Reports 44, 116249, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins NP-40 Beyotime P0013F TRIzol Invitrogen N/A CellTrace CFSE Invitrogen C34554 Lysing Buffer BD Pharm Lyse 555899 CD8 MicroBeads(human) Miltenyi Biotec 130-045-201 Ultra-LEAF Purified anti-human CD3 Biolegend 317325 MACS BSA Stock Solution Miltenyi Biotec 130-091-376 AutoMACS Rinsing Solution Miltenyi Biotec 130-091-222 Ficoll-Paque Plus GE Healthcare 17-1440-02 2×Taq plus master mix (dye plus) Takara RR902Q Taq DNA Polymerase Takara R001A ECL Solution Tanon 180–5001 Dynabeads TM protein G Invitrogen 10003D 5× DNA Loading Yeasen 10507 100 bp DNA Ladder Takara 3422A Fixation/Permeabilization Buffer R&D systems FC007 Glucosamine MedChemExpress HY-B1125 6-diazo-5-oxygen-L-noleucine (DON) Sigma Aldrich D2141 Azoxymethane(AOM) Wako Pure Chemical Industries, Ltd 011–20171 Dextran sulfate sodium salt(DSS) Meilunbio MB5535 FcR Blocking Reagent Miltenyi Biotec 130-C92-575 ConA-agarose beads Vector Laboratories AL-1003 Critical commercial assays Tumor Dissociation Kit (mouse) Miltenyi Biotec 130-096-730 Annexin V-mCherry Kit Beyotime C1069S HILIC TopTip Glygen N/A High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific 84868 IHC Secondary Antibody Kit Absin abs957 Mouse Direct PCR Kit Bimake B40015 Dual-Luciferase® Reporter Assay System Promega E1910 Membrane and Cytosol Protein Extraction Kit Beyotime P0033 MinElute PCR kit Qiagen 28004 VECTASTAIN®ABC Vector Laboratories PK-4000 Click-iT® Protein Reaction Buffer Kit Invitrogen C10276 Deposited data Proteomics in wild-type and GFAT1-depleted DLD-1 cells This study iProX database with identifier IPX0012664000 Experimental models: Cell lines DLD-1 This study N/A SW480 This study N/A MC38 Lab of Yunlong Yang N/A Experimental models: Organisms/strains C57BL/6 mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A BALB/c nude mice Shanghai JSJ Laboratory Animal Co.,Ltd N/A Gfpt1 fl/fl mice Shanghai Model Organisms Center, Inc. N/A Vil-Cre mice Shanghai Model Organisms Center, Inc. N/A (Continued on next page) 18 Cell Reports 44, 116249, September 23, 2025 ..

    Incubation:

    Article Title: Müller cells under hydrostatic pressure modulate retinal cell survival via TRPV1/PLCγ1 complex-mediated calcium influx in experimental glaucoma.
    Article Snippet: Correspondence X. Liu, Department of Cataract, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: xhualiu@sohu.com and G. Zhang, Department of Fundus Disease, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: zhang-guoming@163.com

    DNA Fragmentation Assay:

    Article Title: Müller cells under hydrostatic pressure modulate retinal cell survival via TRPV1/PLCγ1 complex-mediated calcium influx in experimental glaucoma.
    Article Snippet: Correspondence X. Liu, Department of Cataract, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: xhualiu@sohu.com and G. Zhang, Department of Fundus Disease, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: zhang-guoming@163.com

    Fluorescence:

    Article Title: Müller cells under hydrostatic pressure modulate retinal cell survival via TRPV1/PLCγ1 complex-mediated calcium influx in experimental glaucoma.
    Article Snippet: Correspondence X. Liu, Department of Cataract, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: xhualiu@sohu.com and G. Zhang, Department of Fundus Disease, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: zhang-guoming@163.com

    Microscopy:

    Article Title: Müller cells under hydrostatic pressure modulate retinal cell survival via TRPV1/PLCγ1 complex-mediated calcium influx in experimental glaucoma.
    Article Snippet: Correspondence X. Liu, Department of Cataract, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: xhualiu@sohu.com and G. Zhang, Department of Fundus Disease, Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, 18 Zetian Road, Futian District, Shenzhen 518040, China Tel: +86 0755-23959519 E-mail: zhang-guoming@163.com



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    Description of the protocols used in the study.

    Journal: PLoS ONE

    Article Title: First Results in the Use of Bovine Ear Notch Tag for Bovine Viral Diarrhoea Virus Detection and Genetic Analysis

    doi: 10.1371/journal.pone.0164451

    Figure Lengend Snippet: Description of the protocols used in the study.

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